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L4146
Sigma
Leptin human
>97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder
| Synonym: | OB |
| CAS Number: | 177404-21-6 |
| MDL number: | MFCD01094205 |
Description
| Analysis Note | The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells. |
| Physical form | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. |
| Biochem/physiol Actions | Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo. Human and mouse leptin share ~84% sequence identity. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | predicted mol wt ~16 kDa |
| Gene Information | human ... LEP(3952) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Casanueva, F.F., and Dieguez, C., Neuroendocrine regulation and actions of leptin. Front. Neuroendocrinol. 20, 317-363, (1999) |
| Marti, A., et al., Leptin: physiological actions. J. Physiol. Biochem. 55, 43-49, (1999) | |
| Baskin, D.G., et al., Leptin sensitive neurons in the hypothalamus. Horm. Metab. Res. 31, 345-350, (1999) | |
| Winters, B., et al., Reduction of obesity, as induced by leptin, reverses endothelial dysfunction in obese (Lep(ob)) mice. J. Appl. Physiol. 89, 2382-2390, (2000) |
